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The expression of both total and phosphorylated forms of signaling pathway proteins CREB, JNK, NFОєB, p38, ERK1 2, AKT, p70S6K, STAT3 and STAT5 and MMP3 was analyzed by measure of fluorescence intensity MFI using a Luminex system cialis with priligy Cells were disrupted by aspiration through a 23 gauge needle and centrifuged at 15, 000 rpm for 10 min at 4 C to remove debris

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